Background in Statistics and General, Organic and Analytical Chemistry.
Traditional lectures supported by PowerPoint slides (24 hours / 3 CFU), aimed at the acquisition of theoretical and practical knowledge, and practical laboratory trainings (12 hours / 1 CFU), aimed at the application of that knowledge and the acquisition of competences for the resolution of technical problems.
- An introduction to Bioanalytical Chemistry (0.30 CFU)
- Basic Electroanalytical Principles (0.60 CFU)
- Enzyme-Based Electrochemical Biosensors (1.00 CFU)
- Non-Electrochemical Transducers-Based Biosensors (0.30 CFU)
- Immunosensors (0.30 CFU)
- “Living” Biosensors (0.20 CFU)
- Perspectives and Applications of Biosensors (0.30 CFU)
- Laboratory training (1.00 CFU)
The main objectives of the present course, aimed at giving professional qualities in the agro-food fields, are the following:
- to supply fundamental information about the working principles of biosensors;
- to supply the fundamental knowledge of basic principles of electroanalytical techniques, used in the development and application of biosensors;
- to make understandable the main problems that have to be faced in the development and application of biosensors.
Knowledge and understanding capability.
The student will have to know the fundamental working principles of the different biosensors, and to be able in understanding and facing fabrication and management problems of these devices.
Ability to apply knowledge and understanding.
The course is aimed at providing to the student tools to apply the possessed knowledge about the biosensors for the monitoring of safety and quality of foodstuffs.
Autonomy of judgment
Through the acquisition of theoretical, practical and management competences, the student will reach an adequate degree of autonomy of judgement in decisional processes of optimization, application and solution of problems relevant to the biosensors.
The development of communication skills will be stimulated during lessons and laboratory trainings through the discussion about the various problems dealing with the theory and application of the various biosensors.
Learning skills will be stimulated with power point presentations and classroom discussions, also aimed at verifying the effective understanding of the topics covered. Learning skills will be also stimulated by giving supplementary didactic materials, such as scientific papers, and manuals or brochures of the main commercial biosensors, in order to allow the development of abilities in understanding technical documents.
- BIOSENSORS - Theory and Applications
Autore: D.G. Buerk
- BIOSENSOR PRINCIPLES AND APPLICATIONS
Autori: L.J. Blum & P.R. Coulet
Editore: M. DEKKER
- Didactic/scientific documents available on the professor’s web page.
Oral Exam: 3 questions on the main topics of the program. It lasts about 30 minutes necessary to evaluate the student’s preparation level and his/her spiking ability and clarity of presentation.
1. An introduction to Bioanalytical Chemistry (0.30 CFU)
Definition and role of biosensors in analytical chemistry; measurement systems for biosensors.
2. Basic Electroanalytical Principles (0.60 CFU)
Cyclic voltammetry, Electroanalytical techniques based on mass transport control.
3. Enzyme-Based Electrochemical Biosensors (1.00 CFU)
Enzymes: Specificity, use and applications; Immobilization techniques; Amperometric biosensors; Factors affecting sensitivity, selectivity and response time of biosensors; Enzyme electrode kinetics; Multi-enzyme biosensors; Mediated amperometric biosensors; Enzyme inhibition based biosensors; Organic phase enzyme biosensors; Miniaturization techniques; Potentiometric biosensors.
4. Non-Electrochemical Transducers-Based Biosensors (0.30 CFU)
Calorimetric biosensors; Piezoelectric biosensors; Fibre optic biosensors.
5. Immunosensors (0.30 CFU)
Non-labeled and labeled immunosensors; Potentiometric immunosensors; Fibre optic immunosensors; Amperometric immunosensors; Piezoelectric immunosensors.
6. “Living” Biosensors (0.20 CFU)
Microorganisms and bioluminescent bacteria based biosensors; Yeast based biosensors; Cultivated cells and vegetable or animal tissue based biosensors; Receptors based biosensors
7. Perspectives and Applications of Biosensors (0.30 CFU)
Biosensors for food and environmental control; Detection and quantification of microorganisms.
Laboratory training (1.00 CFU)
- Application of the main techniques used in the field of amperometric biosensor;
- Fabrication of a glucose biosensor and evaluation of its performances.